Optimized protocols for RNA-induced silencing complex assembly and cleavage in cultured Drosophila cells

Optimized protocols for RNA-induced silencing complex assembly and cleavage in cultured Drosophila cells

Summary: Here, we provide an optimized RNA-induced silencing complex (RISC) assembly and cleavage protocol in vitro without using radiolabeled RNA. The protocol is useful to characterize the biochemical properties of the RISC. We describe the preparation of RNA probes, the target RNA, and Drosophila...

Full description

Bibliographic Details
Main Authors: Yajie Gao, Yuanxiang Zhu, Qinmiao Sun, Dahua Chen
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell-based Assays
Molecular Biology
Gene Expression
Molecular/Chemical Probes
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004762

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722004762

Similar Items