Female bias in Rhox6 and 9 regulation by the histone demethylase KDM6A.
The Rhox cluster on the mouse X chromosome contains reproduction-related homeobox genes expressed in a sexually dimorphic manner. We report that two members of the Rhox cluster, Rhox6 and 9, are regulated by de-methylation of histone H3 at lysine 27 by KDM6A, a histone demethylase with female-biased...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-05-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3642083?pdf=render |
| _version_ | 1818906800215293952 |
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| author | Joel B Berletch Xinxian Deng Di Kim Nguyen Christine M Disteche |
| author_facet | Joel B Berletch Xinxian Deng Di Kim Nguyen Christine M Disteche |
| author_sort | Joel B Berletch |
| collection | DOAJ |
| description | The Rhox cluster on the mouse X chromosome contains reproduction-related homeobox genes expressed in a sexually dimorphic manner. We report that two members of the Rhox cluster, Rhox6 and 9, are regulated by de-methylation of histone H3 at lysine 27 by KDM6A, a histone demethylase with female-biased expression. Consistent with other homeobox genes, Rhox6 and 9 are in bivalent domains prior to embryonic stem cell differentiation and thus poised for activation. In female mouse ES cells, KDM6A is specifically recruited to Rhox6 and 9 for gene activation, a process inhibited by Kdm6a knockdown in a dose-dependent manner. In contrast, KDM6A occupancy at Rhox6 and 9 is low in male ES cells and knockdown has no effect on expression. In mouse ovary where Rhox6 and 9 remain highly expressed, KDM6A occupancy strongly correlates with expression. Our study implicates Kdm6a, a gene that escapes X inactivation, in the regulation of genes important in reproduction, suggesting that KDM6A may play a role in the etiology of developmental and reproduction-related effects of X chromosome anomalies. |
| first_indexed | 2024-12-19T21:44:59Z |
| format | Article |
| id | doaj.art-714924d8ee8c4257aa365af23b2d2060 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-19T21:44:59Z |
| publishDate | 2013-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-714924d8ee8c4257aa365af23b2d20602022-12-21T20:04:33ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042013-05-0195e100348910.1371/journal.pgen.1003489Female bias in Rhox6 and 9 regulation by the histone demethylase KDM6A.Joel B BerletchXinxian DengDi Kim NguyenChristine M DistecheThe Rhox cluster on the mouse X chromosome contains reproduction-related homeobox genes expressed in a sexually dimorphic manner. We report that two members of the Rhox cluster, Rhox6 and 9, are regulated by de-methylation of histone H3 at lysine 27 by KDM6A, a histone demethylase with female-biased expression. Consistent with other homeobox genes, Rhox6 and 9 are in bivalent domains prior to embryonic stem cell differentiation and thus poised for activation. In female mouse ES cells, KDM6A is specifically recruited to Rhox6 and 9 for gene activation, a process inhibited by Kdm6a knockdown in a dose-dependent manner. In contrast, KDM6A occupancy at Rhox6 and 9 is low in male ES cells and knockdown has no effect on expression. In mouse ovary where Rhox6 and 9 remain highly expressed, KDM6A occupancy strongly correlates with expression. Our study implicates Kdm6a, a gene that escapes X inactivation, in the regulation of genes important in reproduction, suggesting that KDM6A may play a role in the etiology of developmental and reproduction-related effects of X chromosome anomalies.http://europepmc.org/articles/PMC3642083?pdf=render |
| spellingShingle | Joel B Berletch Xinxian Deng Di Kim Nguyen Christine M Disteche Female bias in Rhox6 and 9 regulation by the histone demethylase KDM6A. PLoS Genetics |
| title | Female bias in Rhox6 and 9 regulation by the histone demethylase KDM6A. |
| title_full | Female bias in Rhox6 and 9 regulation by the histone demethylase KDM6A. |
| title_fullStr | Female bias in Rhox6 and 9 regulation by the histone demethylase KDM6A. |
| title_full_unstemmed | Female bias in Rhox6 and 9 regulation by the histone demethylase KDM6A. |
| title_short | Female bias in Rhox6 and 9 regulation by the histone demethylase KDM6A. |
| title_sort | female bias in rhox6 and 9 regulation by the histone demethylase kdm6a |
| url | http://europepmc.org/articles/PMC3642083?pdf=render |
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