Detection of Four Porcine Enteric Coronaviruses Using CRISPR-Cas12a Combined with Multiplex Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay

Detection of Four Porcine Enteric Coronaviruses Using CRISPR-Cas12a Combined with Multiplex Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay

Porcine enteric coronaviruses have caused immense economic losses to the global pig industry, and pose a potential risk for cross-species transmission. The clinical symptoms of the porcine enteric coronaviruses (CoVs) are similar, making it difficult to distinguish between the specific pathogens by...

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Bibliographic Details
Main Authors: Jiajia Liu, Dagang Tao, Xinquan Chen, Linyuan Shen, Li Zhu, Bingrong Xu, Hailong Liu, Shuhong Zhao, Xinyun Li, Xiangdong Liu, Shengsong Xie, Lili Niu
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:Viruses
Subjects:
porcine enteric coronaviruses
CRISPR/Cas12a
multiplex loop-mediated isothermal amplification
single-strand DNA-FQ reporter
Online Access:https://www.mdpi.com/1999-4915/14/4/833

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https://www.mdpi.com/1999-4915/14/4/833

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