Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure

Summary: We describe a protocol to study inflammatory responses triggered by the mRNA-lipid nanoparticle (LNP) vaccine formulations in skin, muscle, and lung and the adaptive immune responses induced in the draining lymph nodes. Here, we will present how to deliver these reagents through intradermal...

Full description

Bibliographic Details
Main Authors: Sonia Ndeupen, Zhen Qin, Botond Z. Igyártó
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002301