Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure
Summary: We describe a protocol to study inflammatory responses triggered by the mRNA-lipid nanoparticle (LNP) vaccine formulations in skin, muscle, and lung and the adaptive immune responses induced in the draining lymph nodes. Here, we will present how to deliver these reagents through intradermal...
Main Authors: | Sonia Ndeupen, Zhen Qin, Botond Z. Igyártó |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-06-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722002301 |
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