A Cost-Effective <i>Pichia pastoris</i> Cell-Free System Driven by Glycolytic Intermediates Enables the Production of Complex Eukaryotic Proteins

A Cost-Effective <i>Pichia pastoris</i> Cell-Free System Driven by Glycolytic Intermediates Enables the Production of Complex Eukaryotic Proteins

Cell-free systems are particularly attractive for screening applications and the production of difficult-to-express proteins. However, the production of cell lysates is difficult to implement on a larger scale due to large time requirements, cultivation costs, and the supplementation of cell-free re...

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Bibliographic Details
Main Authors: Jeffrey L. Schloßhauer, Srujan Kumar Dondapati, Stefan Kubick, Anne Zemella
Format: Article
Language:English
Published: MDPI AG 2024-01-01
Series:Bioengineering
Subjects:
cell-free protein synthesis
yeast
<i>Pichia pastoris</i>
protein production
energy regeneration
design of experiments
Online Access:https://www.mdpi.com/2306-5354/11/1/92

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https://www.mdpi.com/2306-5354/11/1/92

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