Methods of staining and visualization of sphingolipid enriched and non-enriched plasma membrane regions of <it>Arabidopsis thaliana</it> with fluorescent dyes and lipid analogues

Methods of staining and visualization of sphingolipid enriched and non-enriched plasma membrane regions of <it>Arabidopsis thaliana</it> with fluorescent dyes and lipid analogues

<p>Abstract</p> <p>Background</p> <p>Sterols and Sphingolipids form lipid clusters in the plasma membranes of cell types throughout the animal and plant kingdoms. These lipid domains provide a medium for protein signaling complexes at the plasma membrane and are also ob...

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Bibliographic Details
Main Authors: Blachutzik Jörg O, Demir Fatih, Kreuzer Ines, Hedrich Rainer, Harms Gregory S
Format: Article
Language:English
Published: BMC 2012-08-01
Series:Plant Methods
Subjects:
Protoplasts
Lipid polarization
Lipophilic fluorescent dyes
Laurdan
Sphingolipid
Liquid (dis-) ordered phase
Plasma membrane
Fluorescence microscopy
Online Access:http://www.plantmethods.com/content/8/1/28

Internet

http://www.plantmethods.com/content/8/1/28

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