Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator
Abstract Menaquinone-7 (MK-7) is a kind of vitamin K2 playing an important role in the treatment and prevention of cardiovascular disease, osteoporosis and arterial calcification. The purpose of this study is to establish an adaptive evolution strategy based on a chemical modulator to improve MK-7 b...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-11-01
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| Series: | Bioresources and Bioprocessing |
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| Online Access: | https://doi.org/10.1186/s40643-022-00609-0 |
| _version_ | 1811187009984659456 |
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| author | Bei Zhang Cheng Peng Jianyao Lu Xuechao Hu Lujing Ren |
| author_facet | Bei Zhang Cheng Peng Jianyao Lu Xuechao Hu Lujing Ren |
| author_sort | Bei Zhang |
| collection | DOAJ |
| description | Abstract Menaquinone-7 (MK-7) is a kind of vitamin K2 playing an important role in the treatment and prevention of cardiovascular disease, osteoporosis and arterial calcification. The purpose of this study is to establish an adaptive evolution strategy based on a chemical modulator to improve MK-7 biosynthesis in Bacillus natto. The inhibitor of 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase), glyphosate, was chosen as the chemical modulator to perform the experiments. The final strain ALE-25–40, which was obtained after 40 cycles in 25 mmol/L glyphosate, showed a maximal MK-7 titer of 62 mg/L and MK-7 productivity of 0.42 mg/(L h), representing 2.5 and 3 times the original strain, respectively. Moreover, ALE-25–40 generated fewer spores and showed a higher NADH and redox potential. Furthermore, the mechanism related to the improved performance of ALE-25–40 was investigated by comparative transcriptomics analysis. Genes related to the sporation formation were down-regulated. In addition, several genes related to NADH formation were also up-regulated. This strategy proposed here may provide a new and alternative directive for the industrial production of vitamin K2. Graphical Abstract |
| first_indexed | 2024-04-11T13:55:01Z |
| format | Article |
| id | doaj.art-71944e00e21943c2923c1a82d24857ce |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-04-11T13:55:01Z |
| publishDate | 2022-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-71944e00e21943c2923c1a82d24857ce2022-12-22T04:20:22ZengSpringerOpenBioresources and Bioprocessing2197-43652022-11-019111210.1186/s40643-022-00609-0Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulatorBei Zhang0Cheng Peng1Jianyao Lu2Xuechao Hu3Lujing Ren4College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityAbstract Menaquinone-7 (MK-7) is a kind of vitamin K2 playing an important role in the treatment and prevention of cardiovascular disease, osteoporosis and arterial calcification. The purpose of this study is to establish an adaptive evolution strategy based on a chemical modulator to improve MK-7 biosynthesis in Bacillus natto. The inhibitor of 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase), glyphosate, was chosen as the chemical modulator to perform the experiments. The final strain ALE-25–40, which was obtained after 40 cycles in 25 mmol/L glyphosate, showed a maximal MK-7 titer of 62 mg/L and MK-7 productivity of 0.42 mg/(L h), representing 2.5 and 3 times the original strain, respectively. Moreover, ALE-25–40 generated fewer spores and showed a higher NADH and redox potential. Furthermore, the mechanism related to the improved performance of ALE-25–40 was investigated by comparative transcriptomics analysis. Genes related to the sporation formation were down-regulated. In addition, several genes related to NADH formation were also up-regulated. This strategy proposed here may provide a new and alternative directive for the industrial production of vitamin K2. Graphical Abstracthttps://doi.org/10.1186/s40643-022-00609-0Menaquinone-7Bacillus nattoAdaptive evolutionGlyphosateEPSE synthase |
| spellingShingle | Bei Zhang Cheng Peng Jianyao Lu Xuechao Hu Lujing Ren Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator Bioresources and Bioprocessing Menaquinone-7 Bacillus natto Adaptive evolution Glyphosate EPSE synthase |
| title | Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator |
| title_full | Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator |
| title_fullStr | Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator |
| title_full_unstemmed | Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator |
| title_short | Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator |
| title_sort | enhancing menaquinone 7 biosynthesis by adaptive evolution of bacillus natto through chemical modulator |
| topic | Menaquinone-7 Bacillus natto Adaptive evolution Glyphosate EPSE synthase |
| url | https://doi.org/10.1186/s40643-022-00609-0 |
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