Cascade-Cas3 facilitates high-accuracy genome engineering in Pseudomonas using phage-encoded homologous recombination

Cascade-Cas3 facilitates high-accuracy genome engineering in Pseudomonas using phage-encoded homologous recombination

Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome editing. We previously developed and utilized a Pseudomonas-specific PEHR system. However, when using the PEHR system for Pseudomonas genome editing, false positives can be a problem. In this study, we combined a...

Full description

Bibliographic Details
Main Authors: Wentao Zheng, Yandong Xia, Xue Wang, Shiqing Gao, Diao Zhou, Jun Fu, Ruijuan Li, Jia Yin
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:Engineering Microbiology
Subjects:
Phage-encoded homologous recombination
Pseudomonas
Cascade-Cas3 system
Genome engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2667370322000376

Internet

http://www.sciencedirect.com/science/article/pii/S2667370322000376

Similar Items