Cascade-Cas3 facilitates high-accuracy genome engineering in Pseudomonas using phage-encoded homologous recombination
Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome editing. We previously developed and utilized a Pseudomonas-specific PEHR system. However, when using the PEHR system for Pseudomonas genome editing, false positives can be a problem. In this study, we combined a...
Main Authors: | Wentao Zheng, Yandong Xia, Xue Wang, Shiqing Gao, Diao Zhou, Jun Fu, Ruijuan Li, Jia Yin |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-12-01
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Series: | Engineering Microbiology |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2667370322000376 |
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