Standardisation of an In-house Multiplex PCR Assay for Identifying Diffusely Adherent E. coli and ESBL Producing Resistant Isolates

Standardisation of an In-house Multiplex PCR Assay for Identifying Diffusely Adherent E. coli and ESBL Producing Resistant Isolates

We aimed to develop a multiplex PCR (mPCR) assay utilizing the afaC gene for the detection of DAEC pathotypes and the blaTEM gene for the identification of ESBL production in clinical E. coli isolates along with an Internal Amplification Control (IAC) to rule out false negative test results. Monople...

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Bibliographic Details
Main Authors: Radhika Kishor Madalgi, Sughosh Kulkarni, Ganavalli Subramanya Ajantha, Shylaja Ramlal, Shruthi Aradhya, R.D. Kulkarni
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2024-03-01
Series:Journal of Pure and Applied Microbiology
Subjects:
diffusely adherent escherichia coli (daec)
extended spectrum β-lactamase (esbl)
internal amplification control (iac)
multiplex pcr (mpcr)
afac gene
blatem gene
Online Access:https://microbiologyjournal.org/standardisation-of-an-in-house-multiplex-pcr-assay-for-identifying-diffusely-adherent-e-coli-and-esbl-producing-resistant-isolates/

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https://microbiologyjournal.org/standardisation-of-an-in-house-multiplex-pcr-assay-for-identifying-diffusely-adherent-e-coli-and-esbl-producing-resistant-isolates/

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