Inactivation of N-Acetylglucosaminyltransferase I and α1,3-Fucosyltransferase Genes in Nicotiana tabacum BY-2 Cells Results in Glycoproteins With Highly Homogeneous, High-Mannose N-Glycans

Inactivation of N-Acetylglucosaminyltransferase I and α1,3-Fucosyltransferase Genes in Nicotiana tabacum BY-2 Cells Results in Glycoproteins With Highly Homogeneous, High-Mannose N-Glycans

Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells are among the most commonly used plant cell lines for producing biopharmaceutical glycoproteins. Recombinant glycoproteins are usually produced with a mix of high-mannose and complex N-glycans. However, N-glycan heterogeneity is a concern for...

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Bibliographic Details
Main Authors: Xavier Herman, Johann Far, Adeline Courtoy, Laurent Bouhon, Loïc Quinton, Edwin De Pauw, François Chaumont, Catherine Navarre
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-01-01
Series:Frontiers in Plant Science
Subjects:
glyco-engineering
plant suspension cells
oligomannose N-glycans
CRISPR/Cas9
molecular farming
N-acetylglucosaminyltransferase I
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.634023/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2021.634023/full

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