Chromatographic column with nitrile sorbent USED in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatography
The possibility of using chromatographic columns Zorbax SB CN 150´4,6 mm (5 μm) for separation of hydroxycarbamide (hydroxyurea) and its impurity urea has been investigated. The mixture of acetonitrile and water has been used as a mobile phase used. Separation of analytes on nitrile columns under th...
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| Format: | Article |
| Language: | Russian |
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NEICON ISP LLC
2018-02-01
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| Series: | Ведомости Научного центра экспертизы средств медицинского применения |
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| Online Access: | https://www.vedomostincesmp.ru/jour/article/view/95 |
| _version_ | 1797199060698923008 |
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| author | A. S. Osipov E. B. Nechaeva L. A. Truhacheva |
| author_facet | A. S. Osipov E. B. Nechaeva L. A. Truhacheva |
| author_sort | A. S. Osipov |
| collection | DOAJ |
| description | The possibility of using chromatographic columns Zorbax SB CN 150´4,6 mm (5 μm) for separation of hydroxycarbamide (hydroxyurea) and its impurity urea has been investigated. The mixture of acetonitrile and water has been used as a mobile phase used. Separation of analytes on nitrile columns under the conditions of hydrophilic interaction liquid chromatography is possible only when the content of acetonitrile in the mobile phase is more than 90 %. When performing chromatographic procedure with Zorbax SB CN on nitrile column, the change in elution order of hydroxycarbamide and urea has been detected, compared to the elution order on amino column Zorbax NH2. |
| first_indexed | 2024-03-07T19:18:06Z |
| format | Article |
| id | doaj.art-720cf9039fea45adb17069e15264e55f |
| institution | Directory Open Access Journal |
| issn | 1991-2919 2619-1172 |
| language | Russian |
| last_indexed | 2024-04-24T07:09:45Z |
| publishDate | 2018-02-01 |
| publisher | NEICON ISP LLC |
| record_format | Article |
| series | Ведомости Научного центра экспертизы средств медицинского применения |
| spelling | doaj.art-720cf9039fea45adb17069e15264e55f2024-04-21T11:46:32ZrusNEICON ISP LLCВедомости Научного центра экспертизы средств медицинского применения1991-29192619-11722018-02-0103586193Chromatographic column with nitrile sorbent USED in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatographyA. S. Osipov0E. B. Nechaeva1L. A. Truhacheva2Scientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal ProductsThe possibility of using chromatographic columns Zorbax SB CN 150´4,6 mm (5 μm) for separation of hydroxycarbamide (hydroxyurea) and its impurity urea has been investigated. The mixture of acetonitrile and water has been used as a mobile phase used. Separation of analytes on nitrile columns under the conditions of hydrophilic interaction liquid chromatography is possible only when the content of acetonitrile in the mobile phase is more than 90 %. When performing chromatographic procedure with Zorbax SB CN on nitrile column, the change in elution order of hydroxycarbamide and urea has been detected, compared to the elution order on amino column Zorbax NH2.https://www.vedomostincesmp.ru/jour/article/view/95высокоэффективная жидкостная хроматографияхроматография гидрофильных взаимодействийгидроксикарбамид (гидроксимочевина)нитрильный сорбентфармакопеяhigh-performance liquid chromatographyhydrophilic interaction liquid chromatographyhydroxycarbamide (hydroxyurea)nitrile sorbentpharmacopoeia |
| spellingShingle | A. S. Osipov E. B. Nechaeva L. A. Truhacheva Chromatographic column with nitrile sorbent USED in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatography Ведомости Научного центра экспертизы средств медицинского применения высокоэффективная жидкостная хроматография хроматография гидрофильных взаимодействий гидроксикарбамид (гидроксимочевина) нитрильный сорбент фармакопея high-performance liquid chromatography hydrophilic interaction liquid chromatography hydroxycarbamide (hydroxyurea) nitrile sorbent pharmacopoeia |
| title | Chromatographic column with nitrile sorbent USED in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatography |
| title_full | Chromatographic column with nitrile sorbent USED in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatography |
| title_fullStr | Chromatographic column with nitrile sorbent USED in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatography |
| title_full_unstemmed | Chromatographic column with nitrile sorbent USED in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatography |
| title_short | Chromatographic column with nitrile sorbent USED in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatography |
| title_sort | chromatographic column with nitrile sorbent used in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatography |
| topic | высокоэффективная жидкостная хроматография хроматография гидрофильных взаимодействий гидроксикарбамид (гидроксимочевина) нитрильный сорбент фармакопея high-performance liquid chromatography hydrophilic interaction liquid chromatography hydroxycarbamide (hydroxyurea) nitrile sorbent pharmacopoeia |
| url | https://www.vedomostincesmp.ru/jour/article/view/95 |
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