Anhydrous sol-gel synthesis of TiO2 nanoparticles: Evaluating their impact on protein interactions in biological systems
This study focused on the synthesis of anhydrous sol-gel synthesis of TiO2 nanoparticles using a hydrothermal route. The chemical properties of the synthesized TiO2 nanoparticles were confirmed through Fourier Transform Infrared Spectroscopy (FT-IR) and UV–Vis spectroscopy, highlighting their optica...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Journal of Trace Elements and Minerals |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773050623000678 |
| _version_ | 1797326227552337920 |
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| author | Manka Marycleopha Bachir Yaou Balarabe Irédon Adjama Hassimi Moussa Harishchander Anandaram Moumouni Wagé Abdoul Razak |
| author_facet | Manka Marycleopha Bachir Yaou Balarabe Irédon Adjama Hassimi Moussa Harishchander Anandaram Moumouni Wagé Abdoul Razak |
| author_sort | Manka Marycleopha |
| collection | DOAJ |
| description | This study focused on the synthesis of anhydrous sol-gel synthesis of TiO2 nanoparticles using a hydrothermal route. The chemical properties of the synthesized TiO2 nanoparticles were confirmed through Fourier Transform Infrared Spectroscopy (FT-IR) and UV–Vis spectroscopy, highlighting their optical features. Additionally, Energy Dispersive X-ray Spectrometer (EDX) analysis revealed the high purity of the nanomaterial, while the Transmission Electron Microscope (TEM) displayed spherical particles ranging in size from ≥ 80 nm. Furthermore, X-ray diffraction analysis (XRD), Raman and HRTEM analyses indicated that the nanomaterial exhibits an anatase and rutile structure. When, Hen Egg-White Lysozyme (HEWL) incubated with TiO2 led to notable changes: reduced fluorescence indicating protein unfolding, increased light scattering showing protein aggregation, the presence of amyloid fibrils and hydrophobic regions, and reduced enzyme activity. FTIR spectroscopy revealed amyloid structure formation, while Dynamic Light Scattering confirmed the presence of larger protein oligomers. These findings highlight TiO2's adverse impact on HEWL structure and function, emphasizing the need for further research on nanoparticle toxicity in biological systems, particularly in biomedical applications. |
| first_indexed | 2024-03-08T06:20:23Z |
| format | Article |
| id | doaj.art-721716555b254dc989facc57b268f94a |
| institution | Directory Open Access Journal |
| issn | 2773-0506 |
| language | English |
| last_indexed | 2024-03-08T06:20:23Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Trace Elements and Minerals |
| spelling | doaj.art-721716555b254dc989facc57b268f94a2024-02-04T04:47:12ZengElsevierJournal of Trace Elements and Minerals2773-05062024-03-017100114Anhydrous sol-gel synthesis of TiO2 nanoparticles: Evaluating their impact on protein interactions in biological systemsManka Marycleopha0Bachir Yaou Balarabe1Irédon Adjama2Hassimi Moussa3Harishchander Anandaram4Moumouni Wagé Abdoul Razak5School of Forensic Sciences, National Forensic Sciences University, Sector-09, Gandhinagar 382007, IndiaSchool of Engineering and Technology, National Forensic Sciences University, Sector-09, Gandhinagar 382007, India; Corresponding author.School of Pharmacy, National Forensic Sciences University, Sector-09, Gandhinagar 382007, IndiaEnvironmental Science Department, Faculty of Agronomic Sciences, Boubakar Bâ University of Tillaberi BP: 175 Tillaberi, NigerAmrita School of Artificial Intelligence, Coimbatore, Amrita Vishwa Vidyapeetham, IndiaUniversity Andre Salifou, Faculty of Science and Technology, Department of Exact Sciences, BP: 656 Zinder, NigerThis study focused on the synthesis of anhydrous sol-gel synthesis of TiO2 nanoparticles using a hydrothermal route. The chemical properties of the synthesized TiO2 nanoparticles were confirmed through Fourier Transform Infrared Spectroscopy (FT-IR) and UV–Vis spectroscopy, highlighting their optical features. Additionally, Energy Dispersive X-ray Spectrometer (EDX) analysis revealed the high purity of the nanomaterial, while the Transmission Electron Microscope (TEM) displayed spherical particles ranging in size from ≥ 80 nm. Furthermore, X-ray diffraction analysis (XRD), Raman and HRTEM analyses indicated that the nanomaterial exhibits an anatase and rutile structure. When, Hen Egg-White Lysozyme (HEWL) incubated with TiO2 led to notable changes: reduced fluorescence indicating protein unfolding, increased light scattering showing protein aggregation, the presence of amyloid fibrils and hydrophobic regions, and reduced enzyme activity. FTIR spectroscopy revealed amyloid structure formation, while Dynamic Light Scattering confirmed the presence of larger protein oligomers. These findings highlight TiO2's adverse impact on HEWL structure and function, emphasizing the need for further research on nanoparticle toxicity in biological systems, particularly in biomedical applications.http://www.sciencedirect.com/science/article/pii/S2773050623000678NanoparticlesHen egg-white lysozyme (HEWL)InteractionInfluence |
| spellingShingle | Manka Marycleopha Bachir Yaou Balarabe Irédon Adjama Hassimi Moussa Harishchander Anandaram Moumouni Wagé Abdoul Razak Anhydrous sol-gel synthesis of TiO2 nanoparticles: Evaluating their impact on protein interactions in biological systems Journal of Trace Elements and Minerals Nanoparticles Hen egg-white lysozyme (HEWL) Interaction Influence |
| title | Anhydrous sol-gel synthesis of TiO2 nanoparticles: Evaluating their impact on protein interactions in biological systems |
| title_full | Anhydrous sol-gel synthesis of TiO2 nanoparticles: Evaluating their impact on protein interactions in biological systems |
| title_fullStr | Anhydrous sol-gel synthesis of TiO2 nanoparticles: Evaluating their impact on protein interactions in biological systems |
| title_full_unstemmed | Anhydrous sol-gel synthesis of TiO2 nanoparticles: Evaluating their impact on protein interactions in biological systems |
| title_short | Anhydrous sol-gel synthesis of TiO2 nanoparticles: Evaluating their impact on protein interactions in biological systems |
| title_sort | anhydrous sol gel synthesis of tio2 nanoparticles evaluating their impact on protein interactions in biological systems |
| topic | Nanoparticles Hen egg-white lysozyme (HEWL) Interaction Influence |
| url | http://www.sciencedirect.com/science/article/pii/S2773050623000678 |
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