Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells

Summary: The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 in...

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Bibliographic Details
Main Authors: Gowher Ali, Essam M. Abdelalim
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004932