A fluorescence imaging technique suggests that sweat leakage in the epidermis contributes to the pathomechanism of palmoplantar pustulosis
Abstract Sweat is an essential protection system for the body, but its failure can result in pathologic conditions, including several skin diseases, such as palmoplantar pustulosis (PPP). As reduced intraepidermal E-cadherin expression in skin lesions was confirmed in PPP skin lesions, a role for in...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-50875-x |
| _version_ | 1797363446570811392 |
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| author | Kazuki Yatsuzuka Ryosuke Kawakami Yosuke Niko Teruko Tsuda Kenji Kameda Nobushige Kohri Satoshi Yoshida Ken Shiraishi Jun Muto Hideki Mori Yasuhiro Fujisawa Takeshi Imamura Masamoto Murakami |
| author_facet | Kazuki Yatsuzuka Ryosuke Kawakami Yosuke Niko Teruko Tsuda Kenji Kameda Nobushige Kohri Satoshi Yoshida Ken Shiraishi Jun Muto Hideki Mori Yasuhiro Fujisawa Takeshi Imamura Masamoto Murakami |
| author_sort | Kazuki Yatsuzuka |
| collection | DOAJ |
| description | Abstract Sweat is an essential protection system for the body, but its failure can result in pathologic conditions, including several skin diseases, such as palmoplantar pustulosis (PPP). As reduced intraepidermal E-cadherin expression in skin lesions was confirmed in PPP skin lesions, a role for interleukin (IL)-1-rich sweat in PPP has been proposed, and IL-1 has been implicated in the altered E-cadherin expression observed in both cultured keratinocytes and mice epidermis. For further investigation, live imaging of sweat perspiration on a mouse toe-pad under two-photon excitation microscopy was performed using a novel fluorescent dye cocktail (which we named JSAC). Finally, intraepidermal vesicle formation which is the main cause of PPP pathogenesis was successfully induced using our "LASER-snipe" technique with JSAC. "LASER-snipe" is a type of laser ablation technique that uses two-photon absorption of fluorescent material to destroy a few acrosyringium cells at a pinpoint location in three-dimensional space of living tissue to cause eccrine sweat leakage. These observatory techniques and this mouse model may be useful not only in live imaging for physiological phenomena in vivo such as PPP pathomechanism investigation, but also for the field of functional physiological morphology. |
| first_indexed | 2024-03-08T16:21:30Z |
| format | Article |
| id | doaj.art-7231c8071d3d46ebb2b6b7b3324e793b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T16:21:30Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7231c8071d3d46ebb2b6b7b3324e793b2024-01-07T12:19:23ZengNature PortfolioScientific Reports2045-23222024-01-0114111310.1038/s41598-023-50875-xA fluorescence imaging technique suggests that sweat leakage in the epidermis contributes to the pathomechanism of palmoplantar pustulosisKazuki Yatsuzuka0Ryosuke Kawakami1Yosuke Niko2Teruko Tsuda3Kenji Kameda4Nobushige Kohri5Satoshi Yoshida6Ken Shiraishi7Jun Muto8Hideki Mori9Yasuhiro Fujisawa10Takeshi Imamura11Masamoto Murakami12Department of Dermatology, Ehime University Graduate School of MedicineDepartment of Molecular Medicine for Pathogenesis, Ehime University Graduate School of MedicineResearch and Education Faculty, Multidisciplinary Science Cluster, Interdisciplinary Science Unit, Kochi UniversityDepartment of Dermatology, Ehime University Graduate School of MedicineDepartment of Dermatology, Ehime University Graduate School of MedicineDepartment of Dermatology, Ehime University Graduate School of MedicineDepartment of Dermatology, Ehime University Graduate School of MedicineDepartment of Dermatology, Ehime University Graduate School of MedicineDepartment of Dermatology, Ehime University Graduate School of MedicineDepartment of Dermatology, Ehime University Graduate School of MedicineDepartment of Dermatology, Ehime University Graduate School of MedicineDepartment of Molecular Medicine for Pathogenesis, Ehime University Graduate School of MedicineDepartment of Dermatology, Ehime University Graduate School of MedicineAbstract Sweat is an essential protection system for the body, but its failure can result in pathologic conditions, including several skin diseases, such as palmoplantar pustulosis (PPP). As reduced intraepidermal E-cadherin expression in skin lesions was confirmed in PPP skin lesions, a role for interleukin (IL)-1-rich sweat in PPP has been proposed, and IL-1 has been implicated in the altered E-cadherin expression observed in both cultured keratinocytes and mice epidermis. For further investigation, live imaging of sweat perspiration on a mouse toe-pad under two-photon excitation microscopy was performed using a novel fluorescent dye cocktail (which we named JSAC). Finally, intraepidermal vesicle formation which is the main cause of PPP pathogenesis was successfully induced using our "LASER-snipe" technique with JSAC. "LASER-snipe" is a type of laser ablation technique that uses two-photon absorption of fluorescent material to destroy a few acrosyringium cells at a pinpoint location in three-dimensional space of living tissue to cause eccrine sweat leakage. These observatory techniques and this mouse model may be useful not only in live imaging for physiological phenomena in vivo such as PPP pathomechanism investigation, but also for the field of functional physiological morphology.https://doi.org/10.1038/s41598-023-50875-x |
| spellingShingle | Kazuki Yatsuzuka Ryosuke Kawakami Yosuke Niko Teruko Tsuda Kenji Kameda Nobushige Kohri Satoshi Yoshida Ken Shiraishi Jun Muto Hideki Mori Yasuhiro Fujisawa Takeshi Imamura Masamoto Murakami A fluorescence imaging technique suggests that sweat leakage in the epidermis contributes to the pathomechanism of palmoplantar pustulosis Scientific Reports |
| title | A fluorescence imaging technique suggests that sweat leakage in the epidermis contributes to the pathomechanism of palmoplantar pustulosis |
| title_full | A fluorescence imaging technique suggests that sweat leakage in the epidermis contributes to the pathomechanism of palmoplantar pustulosis |
| title_fullStr | A fluorescence imaging technique suggests that sweat leakage in the epidermis contributes to the pathomechanism of palmoplantar pustulosis |
| title_full_unstemmed | A fluorescence imaging technique suggests that sweat leakage in the epidermis contributes to the pathomechanism of palmoplantar pustulosis |
| title_short | A fluorescence imaging technique suggests that sweat leakage in the epidermis contributes to the pathomechanism of palmoplantar pustulosis |
| title_sort | fluorescence imaging technique suggests that sweat leakage in the epidermis contributes to the pathomechanism of palmoplantar pustulosis |
| url | https://doi.org/10.1038/s41598-023-50875-x |
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