Unfolding and Aggregation of Lysozyme under the Combined Action of Dithiothreitol and Guanidine Hydrochloride: Optical Studies

Unfolding and Aggregation of Lysozyme under the Combined Action of Dithiothreitol and Guanidine Hydrochloride: Optical Studies

Using a number of optical techniques (interferometry, dynamic light scattering, and spectroscopy), denaturation of hen egg white lysozyme (HEWL) by treatment with a combination of dithiothreitol (DTT) and guanidine hydrochloride (GdnHCl) has been investigated. The denaturing solutions were selected...

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Bibliographic Details
Main Authors: Ruslan M. Sarimov, Vladimir N. Binhi, Tatiana A. Matveeva, Nikita V. Penkov, Sergey V. Gudkov
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:International Journal of Molecular Sciences
Subjects:
protein hydration shells
optical methods in biochemistry
interferometry
dynamic light scattering (DLS)
UV/visible absorbance spectroscopy
analysis of protein activity
Online Access:https://www.mdpi.com/1422-0067/22/5/2710

Internet

https://www.mdpi.com/1422-0067/22/5/2710

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