Rapid Differential Detection of Wild-Type Classical Swine Fever Virus and Hog Cholera Lapinized Virus Vaccines by TaqMan MGB-Based Dual One-Step Real-Time RT-PCR

Rapid Differential Detection of Wild-Type Classical Swine Fever Virus and Hog Cholera Lapinized Virus Vaccines by TaqMan MGB-Based Dual One-Step Real-Time RT-PCR

To establish a rapid real-time RT-PCR method for differentiating wild-type classical swine fever virus (CSFV) strains from vaccine strains (HCLV), we designed a universal primer targeting the <i>NS3</i> gene to detect wild-type CSFV strains and vaccine strains simultaneously, and two Taq...

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Bibliographic Details
Main Authors: Yongzhe Zhang, Meiqi Wang, Yajuan Sun, Xingyu Xiao, Songsong Wang, Peng Li, Yansong Liu, Hongri Zhao, Yan Meng, Rui Yin
Format: Article
Language:English
Published: MDPI AG 2024-06-01
Series:Veterinary Sciences
Subjects:
CSFV
HCLV
TaqMan-MGB probes
Online Access:https://www.mdpi.com/2306-7381/11/7/289

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https://www.mdpi.com/2306-7381/11/7/289

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