A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR

<p>Abstract</p> <p>Background</p> <p>Based upon defining a common reference point, current real-time quantitative PCR technologies compare relative differences in amplification profile position. As such, absolute quantification requires construction of target-specific s...

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Bibliographic Details
Main Authors: Stewart Don, Rutledge Robert G
Format: Article
Language:English
Published: BMC 2008-05-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/8/47