Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

Abstract Background Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe technology from approximately 150 bp...

Full description

Bibliographic Details
Main Authors: Syukri Shukor, Alfred Tamayo, Lorenzo Tosi, H. Benjamin Larman, Biju Parekkadan
Format: Article
Language:English
Published: BMC 2019-07-01
Series:BMC Biotechnology
Subjects:
Long adapter single-stranded oligonucleotides (LASSO)
Genomic libraries
Multiplex PCR
Multiplex cloning
Online Access:http://link.springer.com/article/10.1186/s12896-019-0547-1

Internet

http://link.springer.com/article/10.1186/s12896-019-0547-1

Similar Items