Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning
Abstract Background Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe technology from approximately 150 bp...
Main Authors: | Syukri Shukor, Alfred Tamayo, Lorenzo Tosi, H. Benjamin Larman, Biju Parekkadan |
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Format: | Article |
Language: | English |
Published: |
BMC
2019-07-01
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Series: | BMC Biotechnology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12896-019-0547-1 |
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