Mind your tag in single-molecule measurements

In this issue of Cell Reports Methods, Molina and colleagues use in vitro single-molecule DNA flow-stretching to demonstrate the severe effects of appending a short lysine-cysteine-lysine (KCK) tag on the Bacillus subtilis ParB protein. This assay could be further utilized to evaluate the impact of...

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Bibliographic Details
Main Authors: Raquel Merino Urteaga, Taekjip Ha
Format: Article
Language:English
Published: Elsevier 2023-10-01
Series:Cell Reports: Methods
Online Access:http://www.sciencedirect.com/science/article/pii/S2667237523002874