Long-term storage of feces at −80 °C versus −20 °C is negligible for 16S rRNA amplicon profiling of the equine bacterial microbiome

The development of next-generation sequencing technologies has spurred a surge of research on bacterial microbiome diversity and function. But despite the rapid growth of the field, many uncertainties remain regarding the impact of differing methodologies on downstream results. Sample storage temper...

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Bibliographic Details
Main Authors: Stefan Gavriliuc, Mason R. Stothart, Astrid Henry, Jocelyn Poissant
Format: Article
Language:English
Published: PeerJ Inc. 2021-03-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/10837.pdf