Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6
Upon target RNA recognition, type III CRISPR-Cas systems produce cyclic oligoadenylates that activate effectors such as Csm6 ribonucleases. Here, Garcia-Doval et al. show that Enteroccocus italicus Csm6 degrades its cyclic hexa-AMP activator, and report the crystal structure of the protein bound to...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-15334-5 |
| _version_ | 1818993886706532352 |
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| author | Carmela Garcia-Doval Frank Schwede Christian Berk Jakob T. Rostøl Ole Niewoehner Oliver Tejero Jonathan Hall Luciano A. Marraffini Martin Jinek |
| author_facet | Carmela Garcia-Doval Frank Schwede Christian Berk Jakob T. Rostøl Ole Niewoehner Oliver Tejero Jonathan Hall Luciano A. Marraffini Martin Jinek |
| author_sort | Carmela Garcia-Doval |
| collection | DOAJ |
| description | Upon target RNA recognition, type III CRISPR-Cas systems produce cyclic oligoadenylates that activate effectors such as Csm6 ribonucleases. Here, Garcia-Doval et al. show that Enteroccocus italicus Csm6 degrades its cyclic hexa-AMP activator, and report the crystal structure of the protein bound to an activator mimic. |
| first_indexed | 2024-12-20T20:49:11Z |
| format | Article |
| id | doaj.art-72db17b42bde4a3aab3bcb1de11e229a |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-20T20:49:11Z |
| publishDate | 2020-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-72db17b42bde4a3aab3bcb1de11e229a2022-12-21T19:26:57ZengNature PortfolioNature Communications2041-17232020-03-011111910.1038/s41467-020-15334-5Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6Carmela Garcia-Doval0Frank Schwede1Christian Berk2Jakob T. Rostøl3Ole Niewoehner4Oliver Tejero5Jonathan Hall6Luciano A. Marraffini7Martin Jinek8Department of Biochemistry, University of ZurichBiolog Life Science Institute GmbH & Co. KGDepartment of Chemistry and Applied Biosciences, Institute for Pharmaceutical Sciences, ETH ZurichLaboratory of Bacteriology, The Rockefeller UniversityDepartment of Biochemistry, University of ZurichDepartment of Biochemistry, University of ZurichDepartment of Chemistry and Applied Biosciences, Institute for Pharmaceutical Sciences, ETH ZurichLaboratory of Bacteriology, The Rockefeller UniversityDepartment of Biochemistry, University of ZurichUpon target RNA recognition, type III CRISPR-Cas systems produce cyclic oligoadenylates that activate effectors such as Csm6 ribonucleases. Here, Garcia-Doval et al. show that Enteroccocus italicus Csm6 degrades its cyclic hexa-AMP activator, and report the crystal structure of the protein bound to an activator mimic.https://doi.org/10.1038/s41467-020-15334-5 |
| spellingShingle | Carmela Garcia-Doval Frank Schwede Christian Berk Jakob T. Rostøl Ole Niewoehner Oliver Tejero Jonathan Hall Luciano A. Marraffini Martin Jinek Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6 Nature Communications |
| title | Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6 |
| title_full | Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6 |
| title_fullStr | Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6 |
| title_full_unstemmed | Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6 |
| title_short | Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6 |
| title_sort | activation and self inactivation mechanisms of the cyclic oligoadenylate dependent crispr ribonuclease csm6 |
| url | https://doi.org/10.1038/s41467-020-15334-5 |
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