PENK inhibits osteosarcoma cell migration by activating the PI3K/Akt signaling pathway
Abstract Background This article reports the effects of proenkephalin (PENK) on osteosarcoma (OS) cell migration. Methods A Gene Expression Omnibus (GEO) dataset was used to identify differentially expressed genes (DEGs) in OS tumor samples and normal human osteoblasts. Tumor tissue and adjacent nor...
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13018-020-01679-6 |
| _version_ | 1798041469051731968 |
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| author | Hai-ping Zhang Zi-liang Yu Bing-bing Wu Fa-rui Sun |
| author_facet | Hai-ping Zhang Zi-liang Yu Bing-bing Wu Fa-rui Sun |
| author_sort | Hai-ping Zhang |
| collection | DOAJ |
| description | Abstract Background This article reports the effects of proenkephalin (PENK) on osteosarcoma (OS) cell migration. Methods A Gene Expression Omnibus (GEO) dataset was used to identify differentially expressed genes (DEGs) in OS tumor samples and normal human osteoblasts. Tumor tissue and adjacent normal tissue were collected from 40 OS patients. MG63 cells were transfected with si-PENK. Transwell migration assays and wound healing assays were performed to compare the effect of PENK on migration. Moreover, LY294002 was used to identify the potential mechanism. Gene expression was examined via qRT-PCR and Western blotting. Results Bioinformatic analysis revealed that PENK was downregulated in OS tumor samples compared with normal human osteoblasts. Moreover, PENK was identified as the hub gene of the DEGs. The PI3K/Akt signaling pathway was significantly enriched in the DEGs. Moreover, PENK was downregulated in OS and MG63 cells compared with the corresponding control cells. Silencing PENK promoted MG63 cell migration; however, treatment with LY294002 partially attenuated PENK silencing-induced OS cell migration. Conclusion PENK inhibits OS cell migration by activating the PI3K/Akt signaling pathway. |
| first_indexed | 2024-04-11T22:21:55Z |
| format | Article |
| id | doaj.art-72f5ba85cf4a48b989fa45ac3d465d11 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T22:21:55Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-72f5ba85cf4a48b989fa45ac3d465d112022-12-22T04:00:06ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2020-04-0115111010.1186/s13018-020-01679-6PENK inhibits osteosarcoma cell migration by activating the PI3K/Akt signaling pathwayHai-ping Zhang0Zi-liang Yu1Bing-bing Wu2Fa-rui Sun3Department of Orthopedics, Second Affiliated Hospital of Nantong UniversityDepartment of Orthopedics, Second Affiliated Hospital of Nantong UniversityDepartment of Orthopedics, Second Affiliated Hospital of Nantong UniversityDepartment of Orthopedics, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare GroupAbstract Background This article reports the effects of proenkephalin (PENK) on osteosarcoma (OS) cell migration. Methods A Gene Expression Omnibus (GEO) dataset was used to identify differentially expressed genes (DEGs) in OS tumor samples and normal human osteoblasts. Tumor tissue and adjacent normal tissue were collected from 40 OS patients. MG63 cells were transfected with si-PENK. Transwell migration assays and wound healing assays were performed to compare the effect of PENK on migration. Moreover, LY294002 was used to identify the potential mechanism. Gene expression was examined via qRT-PCR and Western blotting. Results Bioinformatic analysis revealed that PENK was downregulated in OS tumor samples compared with normal human osteoblasts. Moreover, PENK was identified as the hub gene of the DEGs. The PI3K/Akt signaling pathway was significantly enriched in the DEGs. Moreover, PENK was downregulated in OS and MG63 cells compared with the corresponding control cells. Silencing PENK promoted MG63 cell migration; however, treatment with LY294002 partially attenuated PENK silencing-induced OS cell migration. Conclusion PENK inhibits OS cell migration by activating the PI3K/Akt signaling pathway.http://link.springer.com/article/10.1186/s13018-020-01679-6PENKOsteosarcomaPI3KAktMigration |
| spellingShingle | Hai-ping Zhang Zi-liang Yu Bing-bing Wu Fa-rui Sun PENK inhibits osteosarcoma cell migration by activating the PI3K/Akt signaling pathway Journal of Orthopaedic Surgery and Research PENK Osteosarcoma PI3K Akt Migration |
| title | PENK inhibits osteosarcoma cell migration by activating the PI3K/Akt signaling pathway |
| title_full | PENK inhibits osteosarcoma cell migration by activating the PI3K/Akt signaling pathway |
| title_fullStr | PENK inhibits osteosarcoma cell migration by activating the PI3K/Akt signaling pathway |
| title_full_unstemmed | PENK inhibits osteosarcoma cell migration by activating the PI3K/Akt signaling pathway |
| title_short | PENK inhibits osteosarcoma cell migration by activating the PI3K/Akt signaling pathway |
| title_sort | penk inhibits osteosarcoma cell migration by activating the pi3k akt signaling pathway |
| topic | PENK Osteosarcoma PI3K Akt Migration |
| url | http://link.springer.com/article/10.1186/s13018-020-01679-6 |
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