An attempt to simultaneously quantify the polysaccharide, total lipid, protein and pigment in single Cyclotella cryptica cell by Raman spectroscopy

An attempt to simultaneously quantify the polysaccharide, total lipid, protein and pigment in single Cyclotella cryptica cell by Raman spectroscopy

Abstract Background At present, the conventional methods for determining photosynthetic products of microalgae are usually based on a large number of cell mass to reach the measurement baseline, and the result can only reveal the average state at the population level, which is not feasible for large...

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Bibliographic Details
Main Authors: Xiufen Wang, Yuehui He, Yuanyuan Zhou, Baohua Zhu, Jian Xu, Kehou Pan, Yun Li
Format: Article
Language:English
Published: BMC 2023-04-01
Series:Biotechnology for Biofuels and Bioproducts
Subjects:
Single-cell Raman spectra (SCRS)
Cyclotella cryptica (C. cryptica)
Intracellular energy-dense macromolecules
Partial Least Square Regression (PLSR)
Intra-ramanome Correlation Analysis (IRCA)
Online Access:https://doi.org/10.1186/s13068-023-02314-2

Internet

https://doi.org/10.1186/s13068-023-02314-2

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