Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues
Selection for a promiscuous enzyme activity provides substantial opportunity for competition between endogenous and newly-encountered substrates to influence the evolutionary trajectory, an aspect that is often overlooked in laboratory directed evolution studies. We selected the Escherichia coli nit...
| Главные авторы: | , , , , , , , , , , |
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| Формат: | Статья |
| Язык: | English |
| Опубликовано: |
eLife Sciences Publications Ltd
2020-11-01
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| Серии: | eLife |
| Предметы: | |
| Online-ссылка: | https://elifesciences.org/articles/59081 |