Evaluating the accuracy of amplicon-based microbiome computational pipelines on simulated human gut microbial communities

Evaluating the accuracy of amplicon-based microbiome computational pipelines on simulated human gut microbial communities

Abstract Background Microbiome studies commonly use 16S rRNA gene amplicon sequencing to characterize microbial communities. Errors introduced at multiple steps in this process can affect the interpretation of the data. Here we evaluate the accuracy of operational taxonomic unit (OTU) generation, ta...

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Bibliographic Details
Main Authors: Jonathan L. Golob, Elisa Margolis, Noah G. Hoffman, David N. Fredricks
Format: Article
Language:English
Published: BMC 2017-05-01
Series:BMC Bioinformatics
Subjects:
Microbiome
Classification
Operational taxonomic unit
Optimization
UniFrac
QIIME
Online Access:http://link.springer.com/article/10.1186/s12859-017-1690-0

Internet

http://link.springer.com/article/10.1186/s12859-017-1690-0

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