Optical Clearing Using SeeDB
We describe a water-based optical clearing agent, SeeDB (See Deep Brain), which clears fixed brain samples in a few days without quenching many types of fluorescent dyes, including fluorescent proteins and lipophilic neuronal tracers. SeeDB is a saturated solution of fructose (80.2% w/w) in water wi...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Bio-protocol LLC
2014-02-01
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Series: | Bio-Protocol |
Online Access: | https://bio-protocol.org/en/bpdetail?id=1042&type=0 |