Essential oil and polyphenolics from Thymus satureioides Coss. counteract acrylamide-induced liver toxicity through suppression of NLRP3 inflammasome/NF-κB axis
Acrylamide is a carcinogenic, mutagenic and reprotoxic substance to humans through the intense oxidative stress it induces. As the liver is the most important organ in detoxifying toxic agents, we investigated here the hepatoprotective potential of Thymus satureioides aerial parts extract and their...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623002414 |
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| author | Mona F. Mahmoud Rania A. Elrashidy Heba Osama Mohammed Badreddine Drissi Ismail Mahdi Mansour Sobeh |
| author_facet | Mona F. Mahmoud Rania A. Elrashidy Heba Osama Mohammed Badreddine Drissi Ismail Mahdi Mansour Sobeh |
| author_sort | Mona F. Mahmoud |
| collection | DOAJ |
| description | Acrylamide is a carcinogenic, mutagenic and reprotoxic substance to humans through the intense oxidative stress it induces. As the liver is the most important organ in detoxifying toxic agents, we investigated here the hepatoprotective potential of Thymus satureioides aerial parts extract and their essential oil, profiled by LC–MS/MS and GC–MS, respectively, on acrylamide-induced liver toxicity in vivo. Adult male Wistar albino rats were randomly assigned into six equal groups, namely the negative control group (10% w/v gum acacia and corn oil), the acrylamide group (10% gum acacia and corn oil + acrylamide (25 mg/kg/day)), T. satureioides polyphenols-treated group (100 and 200 mg/kg/day + acrylamide (25 mg/kg/day)), T. satureioides essential oil-treated group (EO, 0.25 ml/kg/day + acrylamide (25 mg/kg/day)), and silymarin group (Positive control) (100 mg/kg/day + acrylamide (25 mg/kg/day)). Liver toxicity was monitored by measuring liver enzyme activities, oxidative stress markers, NLRP3 inflammasome and inflammatory markers, apoptosis signaling, immunohistochemical study and morphometric analysis. Acrylamide administration substantially elevated serum liver enzymes AST, ALT, and serum total bilirubin, altered liver architecture, decreased hepatic GSH, increased hydropic cells scoring, collagen fibre deposition, immunoexpression of liver fibrotic markers (MMP9 and TGF-1β), lipid peroxidation (MDA), inflammatory responses (IL-1β and p38 MAPK) in hepatic tissues, upregulated liver NLRP3 inflammasome activation, and augmented NF-κB and caspase 3 signaling pathways. These toxicity manifestations were significantly counteracted when rats were pretreated with either T. satureioides polyphenolics or EO as well as silymarin, the reference hepatoprotective compound. Both the polyphenolics and EO furnished comparable mitigation effects to those obtained using silymarin. The observed hepatoprotective activities could be attributed to presence of diverse metabolites (EO (39 compounds) and the extract (45 compounds)) with pronounced antioxidant and anti-inflammatory activities. This study strongly shows the effectiveness of T. satureioides polyphenolics and essential oil in mitigating acrylamide-induced liver toxicity. |
| first_indexed | 2024-03-12T16:15:33Z |
| format | Article |
| id | doaj.art-7355f5fc2ecc45cfa796523f94429eeb |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-12T16:15:33Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-7355f5fc2ecc45cfa796523f94429eeb2023-08-09T04:32:25ZengElsevierJournal of Functional Foods1756-46462023-08-01107105641Essential oil and polyphenolics from Thymus satureioides Coss. counteract acrylamide-induced liver toxicity through suppression of NLRP3 inflammasome/NF-κB axisMona F. Mahmoud0Rania A. Elrashidy1Heba Osama Mohammed2Badreddine Drissi3Ismail Mahdi4Mansour Sobeh5Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt; Corresponding authors.Biochemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptHuman Anatomy and Embryology Department, Faculty of Medicine, Zagazig University, Zagazig 44519, EgyptAgrobiosciences Program, College for Sustainable Agriculture and Environmental Science, Mohammed VI Polytechnic University, Ben-Guerir 43150, MoroccoAgrobiosciences Program, College for Sustainable Agriculture and Environmental Science, Mohammed VI Polytechnic University, Ben-Guerir 43150, MoroccoAgrobiosciences Program, College for Sustainable Agriculture and Environmental Science, Mohammed VI Polytechnic University, Ben-Guerir 43150, Morocco; Corresponding authors.Acrylamide is a carcinogenic, mutagenic and reprotoxic substance to humans through the intense oxidative stress it induces. As the liver is the most important organ in detoxifying toxic agents, we investigated here the hepatoprotective potential of Thymus satureioides aerial parts extract and their essential oil, profiled by LC–MS/MS and GC–MS, respectively, on acrylamide-induced liver toxicity in vivo. Adult male Wistar albino rats were randomly assigned into six equal groups, namely the negative control group (10% w/v gum acacia and corn oil), the acrylamide group (10% gum acacia and corn oil + acrylamide (25 mg/kg/day)), T. satureioides polyphenols-treated group (100 and 200 mg/kg/day + acrylamide (25 mg/kg/day)), T. satureioides essential oil-treated group (EO, 0.25 ml/kg/day + acrylamide (25 mg/kg/day)), and silymarin group (Positive control) (100 mg/kg/day + acrylamide (25 mg/kg/day)). Liver toxicity was monitored by measuring liver enzyme activities, oxidative stress markers, NLRP3 inflammasome and inflammatory markers, apoptosis signaling, immunohistochemical study and morphometric analysis. Acrylamide administration substantially elevated serum liver enzymes AST, ALT, and serum total bilirubin, altered liver architecture, decreased hepatic GSH, increased hydropic cells scoring, collagen fibre deposition, immunoexpression of liver fibrotic markers (MMP9 and TGF-1β), lipid peroxidation (MDA), inflammatory responses (IL-1β and p38 MAPK) in hepatic tissues, upregulated liver NLRP3 inflammasome activation, and augmented NF-κB and caspase 3 signaling pathways. These toxicity manifestations were significantly counteracted when rats were pretreated with either T. satureioides polyphenolics or EO as well as silymarin, the reference hepatoprotective compound. Both the polyphenolics and EO furnished comparable mitigation effects to those obtained using silymarin. The observed hepatoprotective activities could be attributed to presence of diverse metabolites (EO (39 compounds) and the extract (45 compounds)) with pronounced antioxidant and anti-inflammatory activities. This study strongly shows the effectiveness of T. satureioides polyphenolics and essential oil in mitigating acrylamide-induced liver toxicity.http://www.sciencedirect.com/science/article/pii/S1756464623002414Thymus satureioidesAcrylamideLiver toxicityCollagen depositionNLRP3 inflammasome/NF-κB axis |
| spellingShingle | Mona F. Mahmoud Rania A. Elrashidy Heba Osama Mohammed Badreddine Drissi Ismail Mahdi Mansour Sobeh Essential oil and polyphenolics from Thymus satureioides Coss. counteract acrylamide-induced liver toxicity through suppression of NLRP3 inflammasome/NF-κB axis Journal of Functional Foods Thymus satureioides Acrylamide Liver toxicity Collagen deposition NLRP3 inflammasome/NF-κB axis |
| title | Essential oil and polyphenolics from Thymus satureioides Coss. counteract acrylamide-induced liver toxicity through suppression of NLRP3 inflammasome/NF-κB axis |
| title_full | Essential oil and polyphenolics from Thymus satureioides Coss. counteract acrylamide-induced liver toxicity through suppression of NLRP3 inflammasome/NF-κB axis |
| title_fullStr | Essential oil and polyphenolics from Thymus satureioides Coss. counteract acrylamide-induced liver toxicity through suppression of NLRP3 inflammasome/NF-κB axis |
| title_full_unstemmed | Essential oil and polyphenolics from Thymus satureioides Coss. counteract acrylamide-induced liver toxicity through suppression of NLRP3 inflammasome/NF-κB axis |
| title_short | Essential oil and polyphenolics from Thymus satureioides Coss. counteract acrylamide-induced liver toxicity through suppression of NLRP3 inflammasome/NF-κB axis |
| title_sort | essential oil and polyphenolics from thymus satureioides coss counteract acrylamide induced liver toxicity through suppression of nlrp3 inflammasome nf κb axis |
| topic | Thymus satureioides Acrylamide Liver toxicity Collagen deposition NLRP3 inflammasome/NF-κB axis |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623002414 |
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