Methods of preparation of fatty acid methyl esters (FAME). Statistical assessment of the precision characteristics from a collaborative trial
The official regulations for the control of the olive and olive pomace oils of the European Union (EU) and International Olive Oil Council (IOOC) include the determination of fatty acids in order to be applied to several purity criteria. The determination of fatty acids require the preparation of th...
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| Format: | Article |
| Language: | English |
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Consejo Superior de Investigaciones Científicas
2000-12-01
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| Series: | Grasas y Aceites |
| Subjects: | |
| Online Access: | http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/464 |
| _version_ | 1818669794335916032 |
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| author | A. Cert W. Moreda M. C. Pérez-Camino |
| author_facet | A. Cert W. Moreda M. C. Pérez-Camino |
| author_sort | A. Cert |
| collection | DOAJ |
| description | The official regulations for the control of the olive and olive pomace oils of the European Union (EU) and International Olive Oil Council (IOOC) include the determination of fatty acids in order to be applied to several purity criteria. The determination of fatty acids require the preparation of the fatty acid methyl esters (FAME) for the subsequent analysis by gas chromatography with good precision and reproducibility. Among the methods used in the laboratories of both the industries and the official institutions looking after the olive oil control, the ones selected were: 1) cold methylation with methanolic potash and 2) hot methylation with sodium methylate followed by acidification with sulphuric acid in methanol and heating. A statistical assessment of the precision characteristics were performed on the determination of fatty acids using both methods by a collaborative trial following the directions included in the AOAC regulation (AOAC 1995). In oils with low acidities, the results obtained for both methylation methods were equivalent. However, the olivepomace oil sample (acidity 15.5%) showed significative differences between the fatty acid compositions obtained using both methylation methods. Finally, the methylation with the acidic+basic method did not yield an increase of the trans-isomers of the fatty acids. |
| first_indexed | 2024-12-17T06:57:52Z |
| format | Article |
| id | doaj.art-735d3b0fa0354a50b9660ac01e5c7c5a |
| institution | Directory Open Access Journal |
| issn | 0017-3495 1988-4214 |
| language | English |
| last_indexed | 2024-12-17T06:57:52Z |
| publishDate | 2000-12-01 |
| publisher | Consejo Superior de Investigaciones Científicas |
| record_format | Article |
| series | Grasas y Aceites |
| spelling | doaj.art-735d3b0fa0354a50b9660ac01e5c7c5a2022-12-21T21:59:22ZengConsejo Superior de Investigaciones CientíficasGrasas y Aceites0017-34951988-42142000-12-0151644745610.3989/gya.2000.v51.i6.464460Methods of preparation of fatty acid methyl esters (FAME). Statistical assessment of the precision characteristics from a collaborative trialA. Cert0W. Moreda1M. C. Pérez-Camino2Instituto de la Grasa (C.S.I.C.), Av. Padre García Tejero, 4. 41012 - Sevilla, SpainInstituto de la Grasa (C.S.I.C.), Av. Padre García Tejero, 4. 41012 - Sevilla, SpainInstituto de la Grasa (C.S.I.C.), Av. Padre García Tejero, 4. 41012 - Sevilla, SpainThe official regulations for the control of the olive and olive pomace oils of the European Union (EU) and International Olive Oil Council (IOOC) include the determination of fatty acids in order to be applied to several purity criteria. The determination of fatty acids require the preparation of the fatty acid methyl esters (FAME) for the subsequent analysis by gas chromatography with good precision and reproducibility. Among the methods used in the laboratories of both the industries and the official institutions looking after the olive oil control, the ones selected were: 1) cold methylation with methanolic potash and 2) hot methylation with sodium methylate followed by acidification with sulphuric acid in methanol and heating. A statistical assessment of the precision characteristics were performed on the determination of fatty acids using both methods by a collaborative trial following the directions included in the AOAC regulation (AOAC 1995). In oils with low acidities, the results obtained for both methylation methods were equivalent. However, the olivepomace oil sample (acidity 15.5%) showed significative differences between the fatty acid compositions obtained using both methylation methods. Finally, the methylation with the acidic+basic method did not yield an increase of the trans-isomers of the fatty acids.http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/464collaborative analysisfatty acid methyl esters (fame)methods of preparationolive oilstatistical analysis |
| spellingShingle | A. Cert W. Moreda M. C. Pérez-Camino Methods of preparation of fatty acid methyl esters (FAME). Statistical assessment of the precision characteristics from a collaborative trial Grasas y Aceites collaborative analysis fatty acid methyl esters (fame) methods of preparation olive oil statistical analysis |
| title | Methods of preparation of fatty acid methyl esters (FAME). Statistical assessment of the precision characteristics from a collaborative trial |
| title_full | Methods of preparation of fatty acid methyl esters (FAME). Statistical assessment of the precision characteristics from a collaborative trial |
| title_fullStr | Methods of preparation of fatty acid methyl esters (FAME). Statistical assessment of the precision characteristics from a collaborative trial |
| title_full_unstemmed | Methods of preparation of fatty acid methyl esters (FAME). Statistical assessment of the precision characteristics from a collaborative trial |
| title_short | Methods of preparation of fatty acid methyl esters (FAME). Statistical assessment of the precision characteristics from a collaborative trial |
| title_sort | methods of preparation of fatty acid methyl esters fame statistical assessment of the precision characteristics from a collaborative trial |
| topic | collaborative analysis fatty acid methyl esters (fame) methods of preparation olive oil statistical analysis |
| url | http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/464 |
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