Multi-color dSTORM microscopy in Hormad1-/- spermatocytes reveals alterations in meiotic recombination intermediates and synaptonemal complex structure.

Multi-color dSTORM microscopy in Hormad1-/- spermatocytes reveals alterations in meiotic recombination intermediates and synaptonemal complex structure.

Recombinases RAD51 and its meiosis-specific paralog DMC1 accumulate on single-stranded DNA (ssDNA) of programmed DNA double strand breaks (DSBs) in meiosis. Here we used three-color dSTORM microscopy, and a mouse model with severe defects in meiotic DSB formation and synapsis (Hormad1-/-) to obtain...

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Bibliographic Details
Main Authors: Lieke Koornneef, Johan A Slotman, Esther Sleddens-Linkels, Wiggert A van Cappellen, Marco Barchi, Attila Tóth, Joost Gribnau, Adriaan B Houtsmuller, Willy M Baarends
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-07-01
Series:PLoS Genetics
Online Access:https://doi.org/10.1371/journal.pgen.1010046

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https://doi.org/10.1371/journal.pgen.1010046

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