Detection of cellular redox reactions and antioxidant activity assays
Living organisms are continuously exposed to reactive oxygen species (ROS) during their lifespan. Small amounts of ROS can act as signal transduction molecules. However, excess ROS cause oxidative stress resulting in multiple adverse effects such as protein and lipid oxidation as well as DNA damage....
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-10-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617304681 |
| _version_ | 1818723057209966592 |
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| author | Dan Meng Peng Zhang Lingling Zhang Hong Wang Chi-Tang Ho Shiming Li Fereidoon Shahidi Hui Zhao |
| author_facet | Dan Meng Peng Zhang Lingling Zhang Hong Wang Chi-Tang Ho Shiming Li Fereidoon Shahidi Hui Zhao |
| author_sort | Dan Meng |
| collection | DOAJ |
| description | Living organisms are continuously exposed to reactive oxygen species (ROS) during their lifespan. Small amounts of ROS can act as signal transduction molecules. However, excess ROS cause oxidative stress resulting in multiple adverse effects such as protein and lipid oxidation as well as DNA damage. Cells have developed an antioxidant defense system to maintain the balance between ROS production and scavenging, but imbalance often occurs leading to excess ROS. In this review, we summarized cellular redox reactions and their detection as well as cellular based assays that are used to measure and reflect the real case scenario of antioxidant potential of compounds of interest. Multiple cellular assays developed to evaluate the cellular levels of ROS and antioxidant activity were also reviewed. These studies lead to better understanding of redox homeostasis and identification of more natural antioxidant products that benefit human health. |
| first_indexed | 2024-12-17T21:04:28Z |
| format | Article |
| id | doaj.art-737d59687eec4cd3bfba5c42b1578d78 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T21:04:28Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-737d59687eec4cd3bfba5c42b1578d782022-12-21T21:32:38ZengElsevierJournal of Functional Foods1756-46462017-10-0137467479Detection of cellular redox reactions and antioxidant activity assaysDan Meng0Peng Zhang1Lingling Zhang2Hong Wang3Chi-Tang Ho4Shiming Li5Fereidoon Shahidi6Hui Zhao7Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, ChinaTianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China; Hubei Key Laboratory for Processing & Application of Catalytic Materials, College of Chemistry & Chemical Engineering, Huanggang Normal University, Hubei, ChinaTianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, ChinaShaklee Corporation, 4747 Willow Road, Pleasanton, CA 94588, USADepartment of Food Science, Rutgers University, New Brunswick, NJ 08901, USAHubei Key Laboratory for Processing & Application of Catalytic Materials, College of Chemistry & Chemical Engineering, Huanggang Normal University, Hubei, China; Corresponding authors.Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, CanadaTianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China; Corresponding authors.Living organisms are continuously exposed to reactive oxygen species (ROS) during their lifespan. Small amounts of ROS can act as signal transduction molecules. However, excess ROS cause oxidative stress resulting in multiple adverse effects such as protein and lipid oxidation as well as DNA damage. Cells have developed an antioxidant defense system to maintain the balance between ROS production and scavenging, but imbalance often occurs leading to excess ROS. In this review, we summarized cellular redox reactions and their detection as well as cellular based assays that are used to measure and reflect the real case scenario of antioxidant potential of compounds of interest. Multiple cellular assays developed to evaluate the cellular levels of ROS and antioxidant activity were also reviewed. These studies lead to better understanding of redox homeostasis and identification of more natural antioxidant products that benefit human health.http://www.sciencedirect.com/science/article/pii/S1756464617304681RedoxROSAntioxidant defense systemCellular antioxidant assayPhytochemicals |
| spellingShingle | Dan Meng Peng Zhang Lingling Zhang Hong Wang Chi-Tang Ho Shiming Li Fereidoon Shahidi Hui Zhao Detection of cellular redox reactions and antioxidant activity assays Journal of Functional Foods Redox ROS Antioxidant defense system Cellular antioxidant assay Phytochemicals |
| title | Detection of cellular redox reactions and antioxidant activity assays |
| title_full | Detection of cellular redox reactions and antioxidant activity assays |
| title_fullStr | Detection of cellular redox reactions and antioxidant activity assays |
| title_full_unstemmed | Detection of cellular redox reactions and antioxidant activity assays |
| title_short | Detection of cellular redox reactions and antioxidant activity assays |
| title_sort | detection of cellular redox reactions and antioxidant activity assays |
| topic | Redox ROS Antioxidant defense system Cellular antioxidant assay Phytochemicals |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617304681 |
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