Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems

Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems

BLIMP1 (PRDM1) and VASA (DDX4) play pivotal roles in the development of the germ cell linage. Importantly, these genes are specifically expressed in germ cells; BLIMP1 in primordial germ cells (PGCs) to early-stage gonocytes, and VASA in migration-stage PGCs to mature gametes. The high reproductive...

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Bibliographic Details
Main Authors: Sho Yoshimatsu, Tsukika Sato, Masafumi Yamamoto, Erika Sasaki, Mayutaka Nakajima, Mari Nakamura, Seiji Shiozawa, Toshiaki Noce, Hideyuki Okano
Format: Article
Language:English
Published: Elsevier 2020-04-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506120300441

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http://www.sciencedirect.com/science/article/pii/S1873506120300441

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