Monitoring of Chlorogenic Acid and Antioxidant Capacity of <em>Solanum melongena</em> L. (Eggplant) under Different Heat and Storage Treatments

<i>Solanum melongena</i> L., also known as eggplant, is a widely consumed vegetable and it is well-known for its beneficial antioxidant properties, due to phenolic compounds. In this work, the influence of different cooking procedures on the content of chlorogenic acid was evaluated on e...

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Main Authors: Petra Šilarová, Lila Boulekbache-Makhlouf, Federica Pellati, Lenka Česlová
Format: Article
Language:English
Published: MDPI AG 2019-07-01
Series:Antioxidants
Subjects:
Online Access:https://www.mdpi.com/2076-3921/8/7/234
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author Petra Šilarová
Lila Boulekbache-Makhlouf
Federica Pellati
Lenka Česlová
author_facet Petra Šilarová
Lila Boulekbache-Makhlouf
Federica Pellati
Lenka Česlová
author_sort Petra Šilarová
collection DOAJ
description <i>Solanum melongena</i> L., also known as eggplant, is a widely consumed vegetable and it is well-known for its beneficial antioxidant properties, due to phenolic compounds. In this work, the influence of different cooking procedures on the content of chlorogenic acid was evaluated on eggplant samples of different geographic origin by high-performance liquid chromatography (HPLC). An easy and quick extraction procedure with 50% methanol as the extraction solvent was optimized for the first time by means of a design-of-experiment and applied to heat treated samples of eggplant. The antioxidant capacity of eggplant extracts was also evaluated by using the ABTS assay and it was correlated with the data obtained by the HPLC method. The content of chlorogenic acid was different in each heat-treated eggplant sample and it depended on the temperature applied during the cooking procedure. In particular, an increase of chlorogenic acid content with rising temperature was observed. Conversely, a very high temperature (250 °C) caused a decrease of chlorogenic acid amount. The influence of storage on the content of chlorogenic acid was also monitored. While the level of chlorogenic acid in fresh samples decreased during four weeks of storage, an increase in its content in heat treated eggplant was observed within the same period. Multivariate data analysis was used to classify eggplant samples into different groups, according to the country of origin and heat treatment procedure. This study provides new insights to preserve the antioxidant properties of eggplant phenolics during different thermal and storage treatments in order to highlight their health promoting effects.
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spelling doaj.art-738c4f48716743cdad03eb91e02430bc2023-09-03T05:52:11ZengMDPI AGAntioxidants2076-39212019-07-018723410.3390/antiox8070234antiox8070234Monitoring of Chlorogenic Acid and Antioxidant Capacity of <em>Solanum melongena</em> L. (Eggplant) under Different Heat and Storage TreatmentsPetra Šilarová0Lila Boulekbache-Makhlouf1Federica Pellati2Lenka Česlová3Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, CZ-53210 Pardubice, Czech RepublicLaboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, AlgeriaDepartment of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, IT-41125 Modena, ItalyDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, CZ-53210 Pardubice, Czech Republic<i>Solanum melongena</i> L., also known as eggplant, is a widely consumed vegetable and it is well-known for its beneficial antioxidant properties, due to phenolic compounds. In this work, the influence of different cooking procedures on the content of chlorogenic acid was evaluated on eggplant samples of different geographic origin by high-performance liquid chromatography (HPLC). An easy and quick extraction procedure with 50% methanol as the extraction solvent was optimized for the first time by means of a design-of-experiment and applied to heat treated samples of eggplant. The antioxidant capacity of eggplant extracts was also evaluated by using the ABTS assay and it was correlated with the data obtained by the HPLC method. The content of chlorogenic acid was different in each heat-treated eggplant sample and it depended on the temperature applied during the cooking procedure. In particular, an increase of chlorogenic acid content with rising temperature was observed. Conversely, a very high temperature (250 °C) caused a decrease of chlorogenic acid amount. The influence of storage on the content of chlorogenic acid was also monitored. While the level of chlorogenic acid in fresh samples decreased during four weeks of storage, an increase in its content in heat treated eggplant was observed within the same period. Multivariate data analysis was used to classify eggplant samples into different groups, according to the country of origin and heat treatment procedure. This study provides new insights to preserve the antioxidant properties of eggplant phenolics during different thermal and storage treatments in order to highlight their health promoting effects.https://www.mdpi.com/2076-3921/8/7/234<i>Solanum melongena</i> L.eggplantcookingHPLCchlorogenic acidantioxidant activity
spellingShingle Petra Šilarová
Lila Boulekbache-Makhlouf
Federica Pellati
Lenka Česlová
Monitoring of Chlorogenic Acid and Antioxidant Capacity of <em>Solanum melongena</em> L. (Eggplant) under Different Heat and Storage Treatments
Antioxidants
<i>Solanum melongena</i> L.
eggplant
cooking
HPLC
chlorogenic acid
antioxidant activity
title Monitoring of Chlorogenic Acid and Antioxidant Capacity of <em>Solanum melongena</em> L. (Eggplant) under Different Heat and Storage Treatments
title_full Monitoring of Chlorogenic Acid and Antioxidant Capacity of <em>Solanum melongena</em> L. (Eggplant) under Different Heat and Storage Treatments
title_fullStr Monitoring of Chlorogenic Acid and Antioxidant Capacity of <em>Solanum melongena</em> L. (Eggplant) under Different Heat and Storage Treatments
title_full_unstemmed Monitoring of Chlorogenic Acid and Antioxidant Capacity of <em>Solanum melongena</em> L. (Eggplant) under Different Heat and Storage Treatments
title_short Monitoring of Chlorogenic Acid and Antioxidant Capacity of <em>Solanum melongena</em> L. (Eggplant) under Different Heat and Storage Treatments
title_sort monitoring of chlorogenic acid and antioxidant capacity of em solanum melongena em l eggplant under different heat and storage treatments
topic <i>Solanum melongena</i> L.
eggplant
cooking
HPLC
chlorogenic acid
antioxidant activity
url https://www.mdpi.com/2076-3921/8/7/234
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AT federicapellati monitoringofchlorogenicacidandantioxidantcapacityofemsolanummelongenaemleggplantunderdifferentheatandstoragetreatments
AT lenkaceslova monitoringofchlorogenicacidandantioxidantcapacityofemsolanummelongenaemleggplantunderdifferentheatandstoragetreatments