Gentamicin Protection Assay to Determine Bacterial Survival within Macrophages

Macrophages are key cells involved in orchestrating host defense against infections. Here, we describe the protocol for a bacterial killing assay in macrophages that can be adapted to any bacterial pathogen. Using this assay, we analyzed the survival of wild-type and mutant strains of Escherichia co...

Full description

Bibliographic Details
Main Authors: Sargurunathan Subashchandrabose, Harry Mobley
Format: Article
Language:English
Published: Bio-protocol LLC 2014-09-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=1235&type=0