Enzymatic Analysis of Yeast Cell Wall-Resident GAPDH and Its Secretion

ABSTRACT In yeast, many proteins are found in both the cytoplasmic and extracellular compartments, and consequently it can be difficult to distinguish nonconventional secretion from cellular leakage. Therefore, we monitored the extracellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity...

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Bibliographic Details
Main Authors: Michael J. Cohen, Brianne Philippe, Peter N. Lipke
Format: Article
Language:English
Published: American Society for Microbiology 2020-12-01
Series:mSphere
Subjects:
Online Access:https://journals.asm.org/doi/10.1128/mSphere.01027-20