Enzymatic Analysis of Yeast Cell Wall-Resident GAPDH and Its Secretion
ABSTRACT In yeast, many proteins are found in both the cytoplasmic and extracellular compartments, and consequently it can be difficult to distinguish nonconventional secretion from cellular leakage. Therefore, we monitored the extracellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity...
Main Authors: | Michael J. Cohen, Brianne Philippe, Peter N. Lipke |
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Format: | Article |
Language: | English |
Published: |
American Society for Microbiology
2020-12-01
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Series: | mSphere |
Subjects: | |
Online Access: | https://journals.asm.org/doi/10.1128/mSphere.01027-20 |
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