Protocol for quantification of the lysosomal degradation of extracellular proteins into mammalian cells

Summary: Endocytic internalization of extracellular proteins plays roles in signaling, nutrient uptake, immunity, and extracellular protein quality control. However, there are few protocols for analyzing the lysosomal degradation of extracellular protein. Here, we purified secreted proteins fused wi...

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Bibliographic Details
Main Authors: Ayaka Tomihari, Momoka Chiba, Akira Matsuura, Eisuke Itakura
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100681X