Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperature
Abstract The study was carried out to investigate the effect of dietary selenium (Se) and vitamin E (VE) supplementation on mRNA level of heat shock proteins, selenoproteins, and antioxidant enzyme activities in the breast meat of broilers under summer heat stress conditions. A total of 200 male bro...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-07-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-018-0641-0 |
| _version_ | 1818939386547404800 |
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| author | Shahnawaz Kumbhar Alam Z. Khan Fahmida Parveen Zaheer A. Nizamani Farman A. Siyal Mohamed E. Abd El-Hack Fang Gan Yunhuan Liu Muhammad Hamid Sonia A. Nido Kehe Huang |
| author_facet | Shahnawaz Kumbhar Alam Z. Khan Fahmida Parveen Zaheer A. Nizamani Farman A. Siyal Mohamed E. Abd El-Hack Fang Gan Yunhuan Liu Muhammad Hamid Sonia A. Nido Kehe Huang |
| author_sort | Shahnawaz Kumbhar |
| collection | DOAJ |
| description | Abstract The study was carried out to investigate the effect of dietary selenium (Se) and vitamin E (VE) supplementation on mRNA level of heat shock proteins, selenoproteins, and antioxidant enzyme activities in the breast meat of broilers under summer heat stress conditions. A total of 200 male broilers (Ross 308) of 1 day age were randomly separated into 4 groups in a complete randomized design and were given a basal diet (Control, 0.08 mg Se/kg diet) or basal diet supplemented with VE (250 mg/kg VE), sodium selenite (0.2 mg/kg Se), or Se + VE (0.2 mg/kg Se + 250 mg/kg VE) to investigate the expression of key antioxidant and heat shock protein (HSP) genes under high temperature stress. Dietary Se, VE and Se + VE significantly enhanced the activities and mRNA levels of catalase as well as superoxide dismutase (SOD) but decreased the mRNA levels of HSP70 and HSP90. Se alone or combined with VE increased the concentration of selenoprotein P and selenoproteins mRNA level and decreased the expression of HSP60. In addition, Se and Se + VE significantly enhanced the glutathione peroxidase (GPx) activity and the expression of GPx1 and GPx4 in breast muscle tissues. It is noteworthy that all the treatments significantly decreased malondialdehyde (MDA) level in the breast meat. Overall results showed that Se in combination with VE has maximal effects to mitigate heat stress. Based on given results it can be recommended that Se + VE are a suitable dietary supplement for broilers to ameliorate the negative effects of summer heat stress conditions. |
| first_indexed | 2024-12-20T06:22:56Z |
| format | Article |
| id | doaj.art-73c60c301ce544b7b7bd91099278d347 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-20T06:22:56Z |
| publishDate | 2018-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-73c60c301ce544b7b7bd91099278d3472022-12-21T19:50:22ZengSpringerOpenAMB Express2191-08552018-07-018111010.1186/s13568-018-0641-0Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperatureShahnawaz Kumbhar0Alam Z. Khan1Fahmida Parveen2Zaheer A. Nizamani3Farman A. Siyal4Mohamed E. Abd El-Hack5Fang Gan6Yunhuan Liu7Muhammad Hamid8Sonia A. Nido9Kehe Huang10Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, College of Veterinary Medicine, Nanjing Agricultural UniversityInstitute of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, College of Veterinary Medicine, Nanjing Agricultural UniversityDepartment of Veterinary Pathology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture UniversityDepartment of Veterinary Pathology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture UniversityDepartment of Animal Nutrition, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture UniversityDepartment of Poultry, Faculty of Agriculture, Zagazig UniversityInstitute of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, College of Veterinary Medicine, Nanjing Agricultural UniversityInstitute of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, College of Veterinary Medicine, Nanjing Agricultural UniversityInstitute of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, College of Veterinary Medicine, Nanjing Agricultural UniversityInstitute of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, College of Veterinary Medicine, Nanjing Agricultural UniversityInstitute of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, College of Veterinary Medicine, Nanjing Agricultural UniversityAbstract The study was carried out to investigate the effect of dietary selenium (Se) and vitamin E (VE) supplementation on mRNA level of heat shock proteins, selenoproteins, and antioxidant enzyme activities in the breast meat of broilers under summer heat stress conditions. A total of 200 male broilers (Ross 308) of 1 day age were randomly separated into 4 groups in a complete randomized design and were given a basal diet (Control, 0.08 mg Se/kg diet) or basal diet supplemented with VE (250 mg/kg VE), sodium selenite (0.2 mg/kg Se), or Se + VE (0.2 mg/kg Se + 250 mg/kg VE) to investigate the expression of key antioxidant and heat shock protein (HSP) genes under high temperature stress. Dietary Se, VE and Se + VE significantly enhanced the activities and mRNA levels of catalase as well as superoxide dismutase (SOD) but decreased the mRNA levels of HSP70 and HSP90. Se alone or combined with VE increased the concentration of selenoprotein P and selenoproteins mRNA level and decreased the expression of HSP60. In addition, Se and Se + VE significantly enhanced the glutathione peroxidase (GPx) activity and the expression of GPx1 and GPx4 in breast muscle tissues. It is noteworthy that all the treatments significantly decreased malondialdehyde (MDA) level in the breast meat. Overall results showed that Se in combination with VE has maximal effects to mitigate heat stress. Based on given results it can be recommended that Se + VE are a suitable dietary supplement for broilers to ameliorate the negative effects of summer heat stress conditions.http://link.springer.com/article/10.1186/s13568-018-0641-0Broiler meatSeleniumVitamin EAntioxidant capacitySelenoproteinsHeat shock proteins |
| spellingShingle | Shahnawaz Kumbhar Alam Z. Khan Fahmida Parveen Zaheer A. Nizamani Farman A. Siyal Mohamed E. Abd El-Hack Fang Gan Yunhuan Liu Muhammad Hamid Sonia A. Nido Kehe Huang Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperature AMB Express Broiler meat Selenium Vitamin E Antioxidant capacity Selenoproteins Heat shock proteins |
| title | Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperature |
| title_full | Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperature |
| title_fullStr | Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperature |
| title_full_unstemmed | Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperature |
| title_short | Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperature |
| title_sort | impacts of selenium and vitamin e supplementation on mrna of heat shock proteins selenoproteins and antioxidants in broilers exposed to high temperature |
| topic | Broiler meat Selenium Vitamin E Antioxidant capacity Selenoproteins Heat shock proteins |
| url | http://link.springer.com/article/10.1186/s13568-018-0641-0 |
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