The analysis base study on mechanical double enzyme technique for isolating and culturing primary chondrocytes

The mechanical-double enzyme method was used in the current study to isolate and culture primary chondrocytes from the chicken growth plates. The feasibility and practicability of the approach were determined by using trypan blue staining, toluidine blue staining, PCR, and flow cytometry. The immuno...

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Bibliographic Details
Main Authors: Wangyuan Yao, Muhammad Fakhar-e-Alam Kulyar, Yanmei Ding, Haitao Du, Yan Zhang, Zhao Zhang, Chuxian Quan, Quan Mo, Jiakui Li
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016123004466