Low impact to fixed cell processing aiming transmission electron microscopy

In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was...

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Bibliographic Details
Main Authors: Ortrud Monika Barth, Marcos Alexandre Nunes da Silva, Debora Ferreira Barreto-Vieira
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 2016-06-01
Series:Memorias do Instituto Oswaldo Cruz
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000600411&lng=en&tlng=en