Single-sister labeling in the C. elegans germline using the nucleotide analog EdU

Single-sister labeling in the C. elegans germline using the nucleotide analog EdU

Summary: Reciprocal exchanges between genetically identical sister chromatids (sister chromatid exchanges or SCEs) have been challenging to study. Here, we describe a protocol that utilizes a pulse/chase of the thymidine analog 5-ethyl-3′-deoxyuridine (EdU) in combination with click chemistry and an...

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Bibliographic Details
Main Authors: David E. Almanzar, Antonia Hamrick, Ofer Rog
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Genetics
Microscopy
Model Organisms
Molecular Biology
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002246

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http://www.sciencedirect.com/science/article/pii/S2666166722002246

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