Production of Bst polymerase for diagnosis of different infections using loop-mediated isothermal amplification

Production of Bst polymerase for diagnosis of different infections using loop-mediated isothermal amplification

Introduction. The large fragment of DNA polymerase I from Geobacillus stearothermophilus GIM1.543 (Bst DNA polymerase) possesses 5'-3' DNA polymerase activity, 5'-3' displacement activity and high processivity. These properties make it possible to use Bst DNA polymerase in loop-m...

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Bibliographic Details
Main Authors: Maria I. Pika, Olga O. Mikheeva, Elena D. Solovyova, Anna V. Valdokhina, Victoria P. Bulanenko, Evgeny A. Cherkashin, Vadim V. Petrov, Kirill V. Krasovitov, Anna S. Cherkashina, Vasily G. Akimkin
Format: Article
Language:Russian
Published: Central Research Institute for Epidemiology 2023-07-01
Series:Журнал микробиологии, эпидемиологии и иммунобиологии
Subjects:
polymerase
isothermal amplification
recombinant enzyme
Online Access:https://microbiol.crie.ru/jour/article/viewFile/15837/1297

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https://microbiol.crie.ru/jour/article/viewFile/15837/1297

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