Screening and validating the optimal panel of housekeeping genes for 4T1 breast carcinoma and metastasis studies in mice

Screening and validating the optimal panel of housekeeping genes for 4T1 breast carcinoma and metastasis studies in mice

Abstract The 4T1 model is extensively employed in murine studies to elucidate the mechanisms underlying the carcinogenesis of triple-negative breast cancer. Molecular biology serves as a cornerstone in these investigations. However, accurate gene expression analyses necessitate data normalization em...

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Bibliographic Details
Main Authors: Jorge Lucas Nascimento Souza, Ana Rafaela Antunes-Porto, Izabela da Silva Oliveira, Chiara Cássia Oliveira Amorim, Luiz Octávio Pires, Isabela de Brito Duval, Luisa Vitor Braga do Amaral, Fernanda Rezende Souza, Evelyn Ane Oliveira, Geovanni Dantas Cassali, Valbert Nascimento Cardoso, Simone Odília Antunes Fernandes, Ricardo Toshio Fujiwara, Remo Castro Russo, Lilian Lacerda Bueno
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Scientific Reports
Subjects:
Housekeeping genes
Gene expression
Experimental metastasis
RT-qPCR
Radioisotopes
Online Access:https://doi.org/10.1038/s41598-024-77126-x

Internet

https://doi.org/10.1038/s41598-024-77126-x

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