Identification of Candidate Genes Associated with Trichothecene Biosynthesis in <i>Fusarium graminearum</i> Species Complex Combined with Transcriptomic and Proteomic Analysis

The <i>Fusarium graminearum</i> species complex is the main causal agent of wheat head blight worldwide. Trichothecenes produced by the pathogen in infected grains have important food safety implications. Previously reported studies on trichothecene production have all focused on the con...

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Main Authors: Jianhua Wang, Shanhai Lin, Guanghui Zhu, Zhiyong Zhao, Haoyu Wang, Miaoping Zhou, Xingming Zhao, Aibo Wu
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/10/8/1479
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author Jianhua Wang
Shanhai Lin
Guanghui Zhu
Zhiyong Zhao
Haoyu Wang
Miaoping Zhou
Xingming Zhao
Aibo Wu
author_facet Jianhua Wang
Shanhai Lin
Guanghui Zhu
Zhiyong Zhao
Haoyu Wang
Miaoping Zhou
Xingming Zhao
Aibo Wu
author_sort Jianhua Wang
collection DOAJ
description The <i>Fusarium graminearum</i> species complex is the main causal agent of wheat head blight worldwide. Trichothecenes produced by the pathogen in infected grains have important food safety implications. Previously reported studies on trichothecene production have all focused on the conditions conducive to mycotoxin production, while the molecular mechanisms of trichothecene biosynthesis in <i>Fusarium</i> strains under normal or non-inducing conditions are still unclear. Here, a global analysis of the fungal gene expression of three strains using the Affymetrix <i>Fusarium</i> GeneChip under non-inducing conditions is reported. Differentially expressed genes were identified among strains with different trichothecene-production ability, and some novel genes associated with trichothecene biosynthesis were found by bioinformatics analysis. To verify the transcriptome results, proteomic analyses of the three strains were conducted under the same culture conditions. In total, 69 unique fungal proteins were identified in 77 protein spots. Combined with transcriptome and proteome analysis, 27 novel genes were predicted to be associated with trichothecene mycotoxin production. A protein, encoded by <i>FGSG_01403</i>, was found to be associated with trichothecene production via proteome analysis. Gene knock-out mutations of <i>FGSG_01403</i> resulted in mutants with increased production of trichothecenes. Future functional analysis of the candidate genes identified in this study may reveal new insights into the negative regulation of trichothecene production in the <i>Fusarium graminearum</i> species complex.
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spelling doaj.art-7420ca8a413d4a218a08c42465ef9d442023-12-03T14:08:44ZengMDPI AGMicroorganisms2076-26072022-07-01108147910.3390/microorganisms10081479Identification of Candidate Genes Associated with Trichothecene Biosynthesis in <i>Fusarium graminearum</i> Species Complex Combined with Transcriptomic and Proteomic AnalysisJianhua Wang0Shanhai Lin1Guanghui Zhu2Zhiyong Zhao3Haoyu Wang4Miaoping Zhou5Xingming Zhao6Aibo Wu7Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai 201403, ChinaInstitute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai 201403, ChinaDepartment of Computer Science and Technology, Tongji University, Shanghai 201804, ChinaInstitute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai 201403, ChinaInstitute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai 201403, ChinaInstitute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai 200433, ChinaSIBS-UGENT-SJTU Joint Laboratory of Mycotoxin Research, CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Shanghai 200031, ChinaThe <i>Fusarium graminearum</i> species complex is the main causal agent of wheat head blight worldwide. Trichothecenes produced by the pathogen in infected grains have important food safety implications. Previously reported studies on trichothecene production have all focused on the conditions conducive to mycotoxin production, while the molecular mechanisms of trichothecene biosynthesis in <i>Fusarium</i> strains under normal or non-inducing conditions are still unclear. Here, a global analysis of the fungal gene expression of three strains using the Affymetrix <i>Fusarium</i> GeneChip under non-inducing conditions is reported. Differentially expressed genes were identified among strains with different trichothecene-production ability, and some novel genes associated with trichothecene biosynthesis were found by bioinformatics analysis. To verify the transcriptome results, proteomic analyses of the three strains were conducted under the same culture conditions. In total, 69 unique fungal proteins were identified in 77 protein spots. Combined with transcriptome and proteome analysis, 27 novel genes were predicted to be associated with trichothecene mycotoxin production. A protein, encoded by <i>FGSG_01403</i>, was found to be associated with trichothecene production via proteome analysis. Gene knock-out mutations of <i>FGSG_01403</i> resulted in mutants with increased production of trichothecenes. Future functional analysis of the candidate genes identified in this study may reveal new insights into the negative regulation of trichothecene production in the <i>Fusarium graminearum</i> species complex.https://www.mdpi.com/2076-2607/10/8/1479<i>Fusarium graminearum</i> species complextrichothecenemycotoxinbiosynthesis
spellingShingle Jianhua Wang
Shanhai Lin
Guanghui Zhu
Zhiyong Zhao
Haoyu Wang
Miaoping Zhou
Xingming Zhao
Aibo Wu
Identification of Candidate Genes Associated with Trichothecene Biosynthesis in <i>Fusarium graminearum</i> Species Complex Combined with Transcriptomic and Proteomic Analysis
Microorganisms
<i>Fusarium graminearum</i> species complex
trichothecene
mycotoxin
biosynthesis
title Identification of Candidate Genes Associated with Trichothecene Biosynthesis in <i>Fusarium graminearum</i> Species Complex Combined with Transcriptomic and Proteomic Analysis
title_full Identification of Candidate Genes Associated with Trichothecene Biosynthesis in <i>Fusarium graminearum</i> Species Complex Combined with Transcriptomic and Proteomic Analysis
title_fullStr Identification of Candidate Genes Associated with Trichothecene Biosynthesis in <i>Fusarium graminearum</i> Species Complex Combined with Transcriptomic and Proteomic Analysis
title_full_unstemmed Identification of Candidate Genes Associated with Trichothecene Biosynthesis in <i>Fusarium graminearum</i> Species Complex Combined with Transcriptomic and Proteomic Analysis
title_short Identification of Candidate Genes Associated with Trichothecene Biosynthesis in <i>Fusarium graminearum</i> Species Complex Combined with Transcriptomic and Proteomic Analysis
title_sort identification of candidate genes associated with trichothecene biosynthesis in i fusarium graminearum i species complex combined with transcriptomic and proteomic analysis
topic <i>Fusarium graminearum</i> species complex
trichothecene
mycotoxin
biosynthesis
url https://www.mdpi.com/2076-2607/10/8/1479
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