Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses

Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses

Gene electrotransfer (GET) is non-viral gene delivery technique, also known as electroporation-mediated gene delivery or electrotransfection. GET is a method used to introduce foreign genetic material (such as DNA or RNA) into cells by applying external pulsed electric fields (PEFs) to create tempor...

Full description

Bibliographic Details
Main Authors: Eivina Radzevičiūtė-Valčiukė, Jovita Gečaitė, Austėja Balevičiūtė, Anna Szewczyk, Augustinas Želvys, Barbora Lekešytė, Veronika Malyško-Ptašinskė, Eglė Mickevičiūtė, Paulina Malakauskaitė, Julita Kulbacka, Vitalij Novickij
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-07-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
transfection
electroporation
GFP
luminescence
in vitro
in vivo
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2024.1430637/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2024.1430637/full

Similar Items