Seamless and orthogonal expression of genetic parts in polyhydroxyalkanoate (PHA)-producing bacterial chassis for plastic bio-upcycling applications

Seamless and orthogonal expression of genetic parts in polyhydroxyalkanoate (PHA)-producing bacterial chassis for plastic bio-upcycling applications

Polyhydroxyalkanoate (PHA)-producing bacteria represent a powerful synthetic biology chassis for waste bioconversion and bio-upcycling where PHAs can be produced as the final products. In this study, we present a seamless plasmid construction for orthogonal expression of recombinant PET hydrolase (P...

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Bibliographic Details
Main Authors: Matthlessa Matthew Minggu, Nur Anisza Hanoum Naseron, Hazlam Shamin Ahmad Shaberi, Nor Azlan Nor Muhammad, Syarul Nataqain Baharum, Ahmad Bazli Ramzi
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:MethodsX
Subjects:
Orthogonal expression
PHA-producing bacteria
Pseudomonas putida
Cupriavidus necator
Seamless cloning
Plastic bio-upcycling
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016123004302

Internet

http://www.sciencedirect.com/science/article/pii/S2215016123004302

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