Reverse Transcription Can Critically Impact the Diagnostic Outcome of <i>BCR::ABL1</i> Quantitative Real-Time RT-PCR

Reverse Transcription Can Critically Impact the Diagnostic Outcome of <i>BCR::ABL1</i> Quantitative Real-Time RT-PCR

Reverse transcriptases (RT) are essential tools in <i>BCR::ABL1</i> fusion transcript monitoring in chronic myeloid leukemia (CML). The RT type and cDNA priming method may impair the stoichiometry of cDNA synthesis, thereby potentially introducing a bias in <i>BCR::ABL1</i> q...

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Bibliographic Details
Main Authors: Birgit Spiess, Helga Kleiner, Irina Tarnopolscaia, Nicole Naumann, Alice Fabarius, Wolf-Karsten Hofmann, Susanne Saussele, Wolfgang Seifarth
Format: Article
Language:English
Published: MDPI AG 2023-08-01
Series:Cancers
Subjects:
chronic myeloid leukemia (CML)
<i>BCR::ABL1</i> monitoring
minimal residual disease
cDNA synthesis
reverse transcriptase
specific priming
Online Access:https://www.mdpi.com/2072-6694/15/15/3914

Internet

https://www.mdpi.com/2072-6694/15/15/3914

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