Influence of cell growth conditions and medium composition on EGFP photostability in live cells

Photostability is a key characteristic of fluorescent proteins. It was recently demonstrated that green fluorescent protein (GFP) photobleaching in live cells can be suppressed by changes in medium composition. Here we show that Ham's F12 medium provides very high enhanced GFP (EGFP) photostabi...

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Bibliographic Details
Main Authors: Anastasia V. Mamontova, Alexey M. Bogdanov, Konstantin A. Lukyanov
Format: Article
Language:English
Published: Taylor & Francis Group 2015-05-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/000114289